AB30. Influence of protein expression in gastric carcinoma cell line BGC823 inhibiting of the ZNF139 by siRNA
Li-Qiao Fan, Yong Li, Lian-Gang Ma, Qun Zhao, Bi-Bo Tan, Zhao-Xing Li, Dong Wang, Yu Liu, Zhi-Dong Zhang, Zhi-Kai Jiao
Objective: To investigate the relationship between ZNF139 gene and biological behavior of gastric carcinoma e.g., proliferation, apoptosis, invasion, metastasis and adhesion.
Methods: (I) Transfect gastric carcinoma cell line BGC823 with ZNF139 specific siRNA and assess the expression of ZNF139 mRNA both before and after the transfection with quantitative RT-PCR; (II) The total protein of the gastric carcinoma cell line were screened and identified by two-dimensional polyacrylamide gel electrophoresis and liquid chromatograph-mass spectrometer (LC-MS); (III) Some identified protein spots were tested by Western blot in gastric carcinoma cell line.
Results: ZNF139 mRNA in gastric carcinoma cell line decreased after transfection and eight significant abnormal expressed protein spots were found. Seven of the protein spots were identified as switch associated protein 70, far upstream element-binding protein 1, Heat shock protein 60, annexin A7, chaperonin-containing tail-less complex protein 1, SUMO1-activating enzyme and annexin A2. The proteins were associated with gastric carcinoma cells’ function e.g., proliferation, apoptosis, invasion, metastasis and adhesion. The expression of those proteins in gastric carcinoma cells identified by Western blot were consistent with the results obtained by proteomics.
Conclusions: The interfering ZNF139 gene in gastric carcinoma cells might affect the expression of multiple genes and their protein products which result to various abnormal gastric carcinoma biological behavior.
Keywords: ZNF139; siRNA; gastric carcinoma; differentially expressed protein; proteomics
