Yong Li, Qun Zhao, Jie Liu, Bi-Bo Tan, Li-Qiao Fan, Qing-Wei Liu, Zhi-Kai Jiao, Xüe-Feng Zhao, Ying-Jie Hao
Objective:
To investigate the effect of TET on the human gastric carcinoma cell line SGC7901 and SGC7901/ADR and its possible mechanism.
Methods:
Cultured SGC7901, SGC7901/ADR were treated with TET (0.5 μg/mL, 1.0 μg/mL, 1.5 μg/mL, 2.0 μg/mL, 2.5 μg/mL), then inhibition rates were measured by MTT assay in vitro. The expressions of ZNF139, MRP-1, MDR1, GST-π were determined by RT-PCR.
Results:
Expressions of ZNF139, MRP-1, MDR1, GST-π mRNA were higher in SGC7901/ADR than in SGC7901. The expressions of ZNF139, MRP-1, MDR1, GST-π were down-regulated in SGC7901/ADR cells efficiently (all P<0.01). Positive correlationship existed between ZNF139 and MRP-1, ZNF139 and MDR1 before treated by TET in SGC7901/ADR, and this relationship was also existed after SGC7901/ADR cells treated by TET.
Conclusions:
TET could achieve MDR reversion in gastric cancer cells by down-regulating the expression of ZNF139, MRP-1, MDR1, GST-π.
