AB76. A peritoneal dissemination xenograft mouse model established for human gastric cancer
Abstract

AB76. A peritoneal dissemination xenograft mouse model established for human gastric cancer

Changhua Zhang1, Lianzhou Chen2, Roderich Schwarz3, Wentao Zeng2, Ying Wu1, Zhewei Wei1, Yulong He1

1The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510080, China;2The Surgery Laboratory, Sun Yat-Sen University, Guangzhou 510080, China;3Division of Surgical Oncology, Simmons Comprehensive Cancer Center, UT Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX 75390, USA

Objective: To establish a stable and convenient peritoneal dissemination mouse model for experimental gastric cancer research.

Methods: Human gastric cancer cell lines AGS, NCI-N87 and SNU-16 were intraperitoneally injected into athytic nude mice and NOD/SCID mice. The peritoneal tumor formation and mouse survival were compared among different groups.

Results: The formation rate of peritoneal tumor in nude mice by intraperitoneal injection of 5×106 AGS, NCI-N87 and SNU16 cells was 0/8,6/8 and 2/8, respectively. The formation rate of peritoneal cancer in SCID mice after implanting 5×106 cells was 0/6 for AGS cell line, 6/6 for NCI-N87 and 6/6 for SNU16. Median mouse survival was 74 days after intraperitoneal injection 10×106 NCI-N87 cells, 95 days for 10×106 SNU16 cells, 78 days for 20×106 SNU16 cells, and 44 days for 40×106 SNU16 cells, respectively.

Conclusions: A peritoneal dissemination mouse model was successfully established by intraperitoneal injection of 20×106-40×106 SNU16 cells and is a reliable and feasible model for gastric cancer research.

Keywords: Gastric cancer; peritoneal dissemination; mouse model; survival

Cite this abstract as: Zhang CH, Chen LZ, Schwarz R, Zeng WT, Wu Y, Wei ZW, He YL. A peritoneal dissemination xenograft mouse model established for human gastric cancer. Transl Gastrointest Cancer 2013;2(S1):AB76. doi: 10.3978/j.issn.2224-4778.2013.s076