52. Expression of cancer stem cell markers in primary lesion and lymphatic metastatic lesions of gastric carcinoma
Original Article

52. Expression of cancer stem cell markers in primary lesion and lymphatic metastatic lesions of gastric carcinoma

Wen-Tao Liu1, Li-Gang Shi1, Jing Xie2, Qiu-Meng Yang1, Min Yan1, Zheng-Gang Zhu1

1Department of Surgery, Ruijin Hospital, Shanghai Jiao Tong University, Shanghai Institute of Digestive Surgery, Shanghai 200025, PR China; 2Department of pathology, Ruijin Hospital, Shanghai Jiao Tong University 201, China

Objective: To study on expressions and clinical significances of cancer stem cell marker CD133, EpCAM, ABCG2, Oct4 in primary lesion and lymphatic metastatic lesions of gastric carcinoma.
Methods: From 2009 to 2011, 36 paraffin samples of gastric carcinoma were obtained. Expressions of four markers were detected in primary lesions (36 cases), in noncancerous gastric mucosa tissue (30 cases) and in lymphatic metastatic lesions (25 cases) by immunohistochemical staining (IHC). Correlations of proteins expressions with clinicopathological parameters were analyzed.
Results: Expressions of CD133, EpCAM, ABCG2 and Oct4 in gastric carcinoma tissues were significantly higher than that of adjacent tissue (P<0.05). Expressions of CD133 was significantly higher in subgroups with severer invasion depth (P=0.029), severer lymph node metastasis (P=0.020) and later TNM stage (P=0.001). Expressions of EpCAM was significantly higher in subgroups with severer invasion depth (P=0.022). Expressions of ABCG2 was significantly higher in subgroups with lower differentiation of tumor (P=0.042), severer invasion depth (P=0.020) and later TNM stage (P=0.035). Expressive rates of CD133, EpCAM and ABCG2 in lymphatic metastatic lesions were 28%, 60% and 44%, lower than that in primary lesion (P<0.05). Expression of EpCAM in lymphatic metastatic lesions was significantly higher in subgroups with higher primary lesion expression (P=0.040).
Conclusions: Expressions of CD133, EpCAM, ABCG2 and Oct4 in gastric carcinoma tissues were significantly higher than that of adjacent tissue. These markers may be regarded as markers of gastric carcinoma. Higher expressive level of CD133 and EpCAM is related with severer advanced gastric cancer. Higher expressive level of ABCG2 is related with lower differentiation of tumor and severer advanced gastric cancer. These four markers express in lymphatic metastatic lesions of gastric carcinoma. Higher expressive level of EpCAM in lymphatic metastatic lesions is associated to higher primary lesion expression.

Key words

Cancer stem cell; primary lesion; lymphatic metastatic lesions; gastric carcinoma

DOI: 10.3978/j.issn.2224-4778.2012.s052