68. The mechanisms of intestinal motility of reconstruction procedures after total gastrectomy for beagles

Objective: This preliminary study was to investigate the physiologic mechanisms of intestinal motility after total gastrectomy.
Methods: Beagles receiving sham operation, FJI or Roux-en-Y after total gastrectomy were sacrificed 48 hours postoperatively. Beagles were gavaged with active carbon migration distance/ the total small intestinal length). Intestinal tissues from the duodenojejunal anastomosis were collected for detecting interstitial cells of Cajal (ICC), inflammation, and apoptosis.
Results: The intestinal transit rate in FJI group was lower than that in Roux-en-Y group, which indicates FJI benefits food storage. More ICC were found in submuocsa of beagles in FJI group than those in Roux-en-Y group. Inflammation in serosal side of the intestine, including hemorrhage, fibrin deposition, and ulceration, neutrophil and macrophage infiltration, and intestinal epithelial cell apoptosis were significant reduced in beagles receiving FJI, than those with Roux-en-Y, which indicates that ameliorating intestinal inflammation and damage may contribute to reducing ICC loss in FJI group.
Conclusions: Clinical beneficial outcomes of intestinal continuity preserved and ameliorating in testinal inflammation and damage may associate whth the reservoir function and preserving intestinal motility.