@article{TGC73,
author = {Tracey A. Martin and Ann-Marie Toms and Leigh Mansel Davies and Shan Cheng and Wen G. Jiang},
title = {The clinical and biological implications of N-WASP expression in human colorectal cancer},
journal = {Translational Gastrointestinal Cancer},
volume = {1},
number = {1},
year = {2011},
keywords = {},
abstract = {Backgrounds: Neural Wiskott-Aldrich Syndrome protein, N-WASP, a member of the WASP family proteins is a regulator of ARP2/3 and cytoskeleton in the cells and has been implicated in regulating cell motility and morphology. N-WASP has been implicated in the development and progression of certain solid tumours. In the present study, we initially investigated the expression levels of N-WASP in a cohort of human colorectal cancers and explored the relationship between N-WASP and clinical outcome. We further examined the impact of N-WASP on the biological functions of colon cancer cells.
Material and methods: A cohort of fresh frozen human colon tissues were used. N-WASP protein in tissues was analysed using an immunohistochemical method. N-WASP transcripts in the tissues were quantified using real-time quantitative PCR methods and correlated with clinical and pathological information of the patients together with clinical outcome. Human colon cancer cell line, HRT18, weakly positive for N-WASP was genetically modified to either over-express N-WASP or to lose N-WASP expression by way of ribozyme transgenes. Cell functions were determined after the genetic manipulation.
Results: Colonic epithelial cells stained positive for N-WASP with the staining mainly in the cytoplasmic region of the cells. However, colon tumour cells had greatly reduced N-WASP staining. The reduction of N-WASP protein was well reflected at message level, in that colon tumour tissues had significantly lower levels of N-WASP transcript compared with normal tissues (p},
url = {https://tgc.amegroups.com/article/view/73}
}