AB28. The influence mechanism of Serpin B1 in cell invasion ability in human gastric carcinoma
Abstract

AB28. The influence mechanism of Serpin B1 in cell invasion ability in human gastric carcinoma

Yu Liu, Yong Li, Qun Zhao, Li-Qiao Fan, Zhi-Dong Zhang, Xue-Feng Zhao, Bi-Bo Tan, Zhi-Kai Jiao, Nan Jia

Department of Gastrointestinal Surgery, the Fourth Affiliated Hospital, Hebei Medical University, Shijiazhuang, Hebei 050011, China

Objective: To investigate Serpin B1 expression in different differentiated gastric carcinoma tissues and cells and its influence on gastric carcinoma cell invasion and to analyze its mechanisms.

Methods: Western blot method was employed for detection of Serpin B1 expression in gastric carcinoma tissue, adjacent tissues and gastric carcinoma cell lines; siRNA was synthesized specific to Serpin B1 and then transfected into gastric carcinoma cell lines BGC823; cell wound healing assay and Transwell chamber invasion assay were used to observe the influence of Serpin B1-siRNA after being transfected into gastric carcinoma cells BGC823 on invasion and migration activity; expression changes in the invasion and migration-related protein expression of MMP-2, MMP-9, ICAM-1 and TIMP-1 were detected before and after transfection.

Results: Serpin B1 expression in gastric adenocarcinoma tissues and cells was significantly higher than the expression in adjacent tissues, and it was significantly increased (P<0.01) with decreasing degree of differentiation. Serpin B1-siRNA after transfection into BGC823 cells significantly inhibited Serpin B1 expression, and the inhibitory effect was the most obvious in the dose concentration of 80 nmol/L and the action time of 48 hours. Serpin B1-siRNA obviously inhibited the invasion and migration activity of gastric carcinoma cell line BGC823. In 48h after Serpin B1-siRNA was transfected into the gastric carcinoma cell line BGC823, the invasion-associated protein expression of MMP-2, of MMP-9, ICAM-1 and the mRNA as well as protein expression and activity were significantly lowered, while TIMP-1 mRNA and protein expression were significantly enhanced.

Conclusions: There are different Serpin B1 expressions in different differentiated gastric carcinoma tissues and cells. Reduced invasion and migration activity of Serpin B1-siRNA after transfection into gastric carcinoma cells may be caused by regulation of balance among the ICAM-1, MMP-2, MMP-9 and TIMP-1.

Keywords: Gastric carcinoma; differentiation; invasion; mechanism; Serpin B1

Cite this abstract as: Liu Y, Li Y, Zhao Q, Fan LQ, Zhang ZD, Zhao XF, Tan BB, Jiao ZK, Jia N. The influence mechanism of Serpin B1 in cell invasion ability in human gastric carcinoma. Transl Gastrointest Cancer 2013;2(S1):AB28. doi: 10.3978/j.issn.2224-4778.2013.s028