AB39. Chemosensitivity of tumor cell of primary tumors and hepatic metastasis in orthotopic implant nude mice models of human gastric carcinoma cells
Abstract

AB39. Chemosensitivity of tumor cell of primary tumors and hepatic metastasis in orthotopic implant nude mice models of human gastric carcinoma cells

Li-Qiao Fan, Yong Li, Qun Zhao, Bi-Bo Tan, Chuan Sun, Zhao-Xing Li, Dong Wang, Yu Liu, Zhi-Dong Zhang, Zhi-Kai Jiao

Department of General Surgery, The Fourth Affiliated Hospital, Hebei Medical University, Shijiazhuang 050011, China

Objective: To investigate the different chemosensitivity of hepatic metastasis (HM) compared to the primary tumor (PT) in orthotopic implant nude mice models of human gastric carcinoma cells.

Methods: Orthopotic implantation nude mice with human gastric carcinoma cell line SGC7901 was established. After formation of HM, sulphorhodamine B (SRB) assay was performed on specimens of PT and HM. The results were then compared with response to 7 antitumor drugs.

Results: Orthopotic implantation nude mice with human gastric carcinoma cells was established successfully. Rate of tumors in orthopotic implantation was 100%, and rate of formation of HM was 75%. The inhibition rates of tumor cells in HM for L-OHP, VCR were lower than those in PT (both P0.05). There was a significantly positive correlation between the inhibition rates of PT and HM for 5-FU, L-OHP, MTX (all P

Conclusions: Heterogeneity of chemosensitivity to antitumor drugs is shown in HM in orthopotic implantation nude mice with hepatic metastasis.

Keywords: Gastric carcinoma; orthopotic implantation nude mice; hepatic metastasis; chemosensitivity

Cite this abstract as: Fan LQ, Li Y, Zhao Q, Tan BB, Sun C, Li ZX, Wang D, Liu Y, Zhang ZD, Jiao ZK. Chemosensitivity of tumor cell of primary tumors and hepatic metastasis in orthotopic implant nude mice models of human gastric carcinoma cells. Transl Gastrointest Cancer 2013;2(S1):AB39. doi: 10.3978/j.issn.2224-4778.2013.s039