AB45. Significance and relationship between ZNF139 mRNA and methylation of RUNX3 in gastric cancer
Abstract

AB45. Significance and relationship between ZNF139 mRNA and methylation of RUNX3 in gastric cancer

Yong Li, Liqiao Fan, Zhaojie An, Qun Zhao, Bibo Tan, Yü Liu, Dong Wang, Zhidong Zhang

Department of Gastrointestinal Surgery, the Fourth Affiliated Hospital, Hebei Medical University, Shijiazhuang 050011, China

Objective: To investigate the expression of Znic finger Protein 139 (ZNF139) and methylation of RUNX3 in gastric cancer and their relationship with clinicopathological characteristics.

Methods: 55 cases of gastric fresh tumor specimens and paired tumor-adjacent tissues were collected, and data of clinicopathological characteristics were also recorded. Expressions of ZNF139 were determined by RT-PCR, and methylation of RUNX3 was tested by methylation-specific PCR (MSP) assay.

Results: Expressions of ZNF139 were stronger in tumors than in tumor-adjacent tissues (P<0.05). Overexpression of ZNF139 was correlated with tumor size, differentiation, tumor invasion depth, lymph node metastasis (all P<0.05). Rate of RUNX3 methylation in gastric cancer (54.55%) was higher than that in tumor-adjacent tissues (7.27%) (P<0.05). Rate of RUNX3 methylation was correlated with differentiation, tumor invasion depth, lymph node metastasis (all P<0.05). Significant relationship was found between ZNF139 mRNA and methylation of RUNX3 (P<0.05).

Conclusions: ZNF139 was overexpressed in gastric cancer, and rate of RUNX3 methylation was up-regulated. They may be involved in progression and metastasis of gastric cancer.

Keywords: Gastric cancer; znic finger protein 139 (ZNF139); human runt-related transcription factor 3; clinicopathological characteristics

Cite this abstract as: Li Y, Fan LQ, An ZJ, Zhao Q, Tan BB, Liu Y, Wang D, Zhang ZD. Significance and relationship between ZNF139 mRNA and methylation of RUNX3 in gastric cancer. Transl Gastrointest Cancer 2013;2(S1):AB45. doi: 10.3978/j.issn.2224-4778.2013.s045