AB46. Mechanism for transcription factor ZNF139’s inhibition of proliferation and apoptosis regulation of gastric cancer cell

Objective: To study siRNA-ZNF139 (small interference RNA-zinc finger protein 139) before and after treatment of cultured human gastric cancer cell line SGC7901 growth, expression of proliferation and apoptosis related factors in different intervention.

Methods: Construct the siRNA recombinant plasmid of targeting ZNF139 gene and import it into the SGC7901 of gastric cancer cells. Draw the time-dose curve of gastric cancer cell transfection and select the optimum transfection dose and time through MTT method by evaluating the ability of the gastric cancer cell transfection with mediated siRNA-ZNF139 and nonspecific siRNA in different doses; observe the apoptosis rate and cell cycle through FCM; study the mRNA expression of ZNF139, Cyclin D1, PCNA, Caspase-3 and Bcl-2 in each treatment group of human gastric cancer cell SGC7901 by RT-PCR method, together with its mechanism on proliferation and apoptosis regulation of gastric cancer cell.

Results: The built siRNA-ZNF139 plasmid had the ability to silence the expression of transcription factor ZNF139 in SGC7901 cell line specifically, inhibit the growth of tumor cell, promote cell apoptosis, and block the cell in Phase G1. In the gastric cancer cell SGC7901/siRNA-ZNF139, the Cyclin D1, PCNA and Bcl-2 expression in general mRNA wa down regulated while Caspase-3 expression was up regulated; ZNF139 expression showed the positive correlation compared with Cyclin D1, PCNA and Bcl-2 but the negative correlation with Caspase-3.

Conclusions: ZNF139 may complete the proliferation and apoptosis regulation of gastric cancer cell by regulating Cyclin D1, PCNA, Caspase-3, Bcl-2 and other associated factors.

Keywords: Zinc finger protein 139; SiRNA; gastric cancer cell; proliferation; apoptosis