AB72. The residue gastric cells after chemotherapy acquire the property of cancer stem cell
Abstract

AB72. The residue gastric cells after chemotherapy in vitro acquire the property of cancer stem cell

Zhi-Yuan Xu1, Xiang-Dong Cheng1, Ge-Er Zhang2, Yi-An Du1, Ling Huang1

1Department of Abdominal tumor surgery, Zhejiang Cancer Hospital, HangZhou, China;2Department of Gastrointestinal Surgery, The First Affiliated Hospital, Zhejiang University, Hangzhou, China

Aim: To investigate the emergence of cancer stem cell features in the residual gastric cancer (GC) cells after chemotherapy in vitro.

Methods: We treated the GC cell, SGC7901, with 5-Fu at 30 μg/mL concentration, the residue cells after 3 cycle of 5-Fu treatment was named SGC7901/Fu. The malignant biological features including morphological change, chemoresistance, characteristics of cancer stem cell were compared between SGC7901 and SGC7901/Fu.

Results: SGC7901/Fu displayed some mesenchymal appearance. The 50% inhibitory concentration (IC50) of SGC7901/Fu was increased significantly when compared to that of SGC7901 (0.46 μg/mL ±45, P<0.05), SGC7901/Fu showed enhanced ability of tumorogenesis (0.46 μg/mL ±45, P<0.05) and clone formation (0.46 μg/mL ±45, P<0.05). The portion of CD44+CD24- cells in SGC7901/Fu were significantly increased when compared to that of SGC7901 (0.46 μg/mL ±45, P<0.05). The expression of some cancer stem cell-related proteins (including ABCG2, KLF4 and BMI-1) was increased in SGC7901/Fu cells.

Conclusions: The residue GC cells after 5-Fu treatment acquired some malignant biological features including chemoresistance, and property of cancer stem cell.

Keywords: gastric cancer (GC); chemoresistance; cancer stem cell; 5-Fu

Cite this abstract as: Xu ZY, Cheng XD, Zhang GE, Du YA, Huang L. The residue gastric cells after chemotherapy in vitro acquire the property of cancer stem cell. Transl Gastrointest Cancer 2013;2(S1):AB72. doi: 10.3978/j.issn.2224-4778.2013.s072