AB93. Expression of Rac1 and HIF-1α and VEGF in gastric carcinoma: correlation with angiogenesis and prognosis
Hongjie Zhan, Han Liang, Xiangyu Liu, Jingyu Deng, Baogui Wang, Xishan Hao
Background and aim: To investigate the relationship between the expression of hypoxia inducible factor-1α (HIF-1α), Ras-related C3 botulinum toxin substrate 1 (Rac1), VEGF, as well as their correlation with angiogenesis and prognosis in gastric carcinoma.
Methods: The expression of Rac1, HIF-1α, VEGF, and CD34 (described in terms of microvessel density, MVD) was determined by immunohistochemical staining of tissues from 60 cases of radical resected gastric cancer specimens.
Results: The proportion specimens that expressed Rac1, HIF-1α and VEGF were 37/60 (61.7%), 35/60 (58.3%) and 40/60 (66.7%). The levels of Rac1, HIF-1α and VEGF expression were significantly correlated with Lauren’s classification, lymph node metastasis, and pathologic staging (P<0.05). There were positive correlations between MVD and the expression of Rac1, HIF-1α and VEGF. The mean survival time and the 5-year survival rate in cases with positive Rac1, HIF-1α and VEGF expression and MVD ≥26.3 were significantly shorter than those with negative Rac1, HIF-1α and VEGF expression and MVD was <26.3.
Conclusions: Rac1, HIF-1α and VEGF play an important role in tumor invasion and metastasis, especially in tumor angiogenesis. Thus, testing the expression of Rac1, HIF-1α and VEGF may be a useful index for treatment and prognosis.
Keywords: Gastrointestinal cancer; Rac1; hypoxia inducible factor-1α; VEGF; microvessel density; prognosis
