AB38. Expression of PARP1 in human colorectal cancer and its relationship with tumor cell proliferation and apoptosis of SW620 cells

Objective: To investigate PARP1 expression in tumor and adjacent normal tissues from colorectal cancer (CRC) patients, and colon cancer lines SW620 and LoVo, and analyze the relationship between PARP1 expression and clinic pathologic features, and the effect of PARP1 expression on proliferation and apoptosis of SW620 cells.

Methods: Immunohistochemistry and qPCR were respectively used to detect PARP1 expression in CRC tissues and adjacent normal intestinal mucosa, and in SW620 and LoVo cells... After transfection with PARP1-shRNA, the proliferation and apoptosis of SW620 cells were detected by MTT and flow cytometry. The gene and protein expressions of cyclinD1 and Caspase-3 were detected by qPCR and Western blotting.

Results: The positive expression rate of PARP1 was 71.8% in CRC tissues, significantly higher than 20.5% in adjacent normal intestinal mucosa tissues (P<0.05). The expression levels of PARP1 in SW620, LoVo cells were significantly higher than that in normal intestinal mucosa cells. After transfection with PARP1-shRNA, PARP1 expression and cell proliferation were significantly inhibited, while tumor cell apoptosis increased.

Conclusions: PARP1 expression is high in CRC, which can promote proliferation and prohibit apoptosis of tumor cells.